The Development of Admetus Pumilio Koch

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Excerpt from The Development of Admetus Pumilio Koch: A Contribution to the Embryology of the PedipalpiIt was at first very difficult to obtain good cuts through the eggs, because of the presence of the, great amount of yolk which they contained. When embedded in paraffin the yolk always crumbled away before the knife of the microtome.The following is the method I usually used: The embryos were brought through successive degrees of alcohol into absolute alcohol. Next they were left in celloidin for some days. They were then taken from the celloidin and put directly into chloroform. This has the double effect of making the celloidin firm, and of rendering the embryos ready to be transferred into paraffin. After having been treated thus, the embryos were cut as ordi nary paraffin objects. The cuts were attached to the object-glass with water, when all the cuts were arranged on the glass, the water was quickly removed with a piece of blotting-paper, whereupon very ¿uid celloidin was poured over the cuts, they were then allowed to dry, but being still enclosed in paraffin the cuts could not shrink in any way. When quite dry the coating of celloidin became very thin indeed.About the PublisherForgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.comThis book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully, any imperfections that remain are intentionally left to preserve the state of such historical works.