Multiple Detection in Size-Exclusion Chromatography
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Multiple Detection in Size-Exclusion Chromatography provides a comprehensive view of detection techniques that are used synergistically in size-exclusion chromatography (SEC). These techniques include differential refractometry, static and dynamic light scattering, differential viscometry, fluorescence, ultraviolet, infrared, and nuclear magnetic resonance spectroscopy, electrospray ionization, matrix-assisted laser desorption ionization, chemical reaction interface, inductively coupled plasma, and tandem mass spectrometry, and dynamic surface tension. This book shows how thesetechniques provide useful molar mass, architectural, compositional, and thermodynamic information whether SEC is used as the sole separation method or as part of a two-dimensional chromatographic set-up. In the latter case, the ability to separate analytes based on size, provided by SEC, isaugmented by separation based on chemical composition, block length, etc., provided by high performance liquid chromatography, liquid chromatography at the critical condition, phase fluctuation chromatography, or high osmotic pressure chromatography. A large number of applications are shown throughout the book, exemplifying both natural and synthetic polymers. These include, but are not restricted to, the study of long- and short-chain branching in polyolefins, the study of methyl methacrylate polymers, copolymers, and oligomers, elementalspeciation in environmental and biological samples, the characterization of oligo- and polysaccharides, the analysis of complex protein mixtures and of glycoproteins and protein-polymer conjugates, the study of polymer reaction kinetics, the determination of chemical andcompositional heterogeneity, the characterization of semi-flexible synthetic polymers and polypeptides, and the study of chromatographic band broadening.
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