Electrofocusing and Isotachophoresis

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Frontmatter -- Preface -- Contents -- Contributors -- Part I. Isoelectric Focusing -- 1. Theoretical and General Aspects -- Reminiscences about the Genesis of Isoelectric Focusing and Generalization of the Idea / Kolin, A. -- Stable pH Gradients - A Key Problem in Isoelectric Focusing / Rilbe, H. -- Electrofocusing in Buffers: Formation of Natural pH Gradients, Flexibility, Gradient Stability, Relation to Isotachophoresis and Preparative Potential / Chrambach, A. / Nguyen, Nga Y. -- Comparison of pH Gradients Used for Isoelectric Focusing / Fawcett, J. S. -- On the Temperature Dependence of Isoelectric Points of Proteins with Special Reference to Isoelectric Focusing / Fredriksson, S. -- 2. Carrier Ampholytes -- Carrier Ampholyte Distribution / Brown, R. K. / Caspers, M. L. / Vinogradow, S. N. -- Characterization of Carrier Ampholytes by New Visualization Reactions and Chromatographic Methods / Radola, B. J. / Tschesche, H. / Schuricht, H. -- Synthesis of Carrier Ampholytes for Isoelectric Focusing Containing Sulfonic and Phosphonic Acid Groups Covering a Wide pH Range / Grubhofer, N. / Borja, C. -- 3. Analytical Methodology -- Staining of Proteins in Polyacrylamide Gels / Vesterberg, O. / Hansén, L. -- Isoelectric Focusing of Complex Protein Mixtures in the Nanogram Range and Enzyme Kinetics of Dehydrogenases Following IEF / Bispink, G. / Neuhoff, V. -- Soft Laser Scanning Densitometer Compatible with the High Resolution Obtained by Electrofocusing / Zeineh, R. A. -- Immunocore Electrofocusing: A Separation and Detection Technique Amenable to Scanning Densitometry / Zeineh, R. A. -- Rapid, Convenient and Economical Procedures for the Determination of the Isoelectric Spectra of Proteins / Leaback, D. H. -- A Simple Method of Choosing Optimum pH-Conditions for Electrophoresis / Rosengren, Å. / Bjellqvist, B. / Gasparic, V. -- pH Determinations in Isoelectric Focusing with an Iridium Electrode / Gianazza, E. / Righetti, P. G. / Bordi, S. / Papeschi, G. -- Anomalous Behaviour of Horseradish Peroxidase in Isoelectric Focusing / Delincée, H. / Radola, B. J. -- Does Lipoprotein Lipase Bind Ampholytes? / Bengtsson, G. / Olivecrona, T. -- 4. Biochemical Applications -- Zein: Macromolecular Properties, Biosynthesis and Genetic Regulation / Righetti, P. G. / Gianazza, E. / Salamini, F. / Galante, E. / Viotti, A. / Soave, C. -- Growth and Isoelectric Patterns of Peroxidase in Tobacco Tissue Cultures Under the Influence of Growth Regulator Systems / Rücker, W. / Markotai, J. -- A Method for Simultaneous Analysis of Several Genetic Loci in Mice After Electrofocusing / Narayanan, K. R. / Ray, A. S. -- Phylogenetic Differences in Isoelectric Components of Liver Acid Phosphatase and LDH Between Genera of Two Closely Related Rodent Families (Muridae and Microtidae) / Kubicz, A. / Wolanska, L. -- Fractionation of Nucleic Acids on Isoelectric Focusing / Drysdale, J. W. -- 5. Clinical Applications -- Polyacrylamide Gel Isoelectric Focusing of Alpha-1-Proteinase Inhibitors (a1 Pi) Phenotypes: I. Optimal Condition, Histochemical and Proteinase Probe Studies / Allen, R. C. / Oulla, P. M. / Arnaud, P. / Baumstark, J. S. -- Polyacrylamide Gel Isoelectric Focusing (PAGIF) Applied to Alpha-1-Proteinase Inhibitor (a1 Pi) Phenotyping: II. Immunochemical Procedures / Arnaud, P. / Chapuis-Cellier, C. / Wilson, G. B. / Koistinen, J. / Allen, R. C. / Fudenberg, H. H. -- High Resolution Phenotyping of a1-Antitrypsins by Thin-Layer Polyacrylamide Electrofocusing / Jeppsson, J. O. -- Broad-Beta Disease (Hyperlipoproteinaemia Type III): Genetics, Gene Frequency and Diagnosis without Ultracentrifugation / Utermann, G. / Hess, M. / Vogelberg, K. H. -- Screening for Abnormal Hemoglobins in the Newborn: A Highly Economic Procedure Using Isoelectric Focusing / Altland, K. -- The Crystallins of the Aging Lens from Five Species Studies by Various Methods of Thin-Layer Isoelectric Focusing / Bours, J. -- Separation of Erythrocyte Membrane Components in Electric Field. Polyacrylamide Gel Isoelectric Focusing: A Potential Method for the Diagnosis of Membrane Abnormality / Das, P. K. / Graesslin, D. / Goedde, H. W. -- Microheterogeneity of Serum Glycoproteins as Revealed by Flat-Bed Gel Isoelectric Focusing / Hamann, A. -- Diagnosis of Cystic Fibrosis (CF) - Purification and Distinction Between CF Protein and Ciliary Dyskinesia Activity in CF and Asthmatic Sera Using Isoelectric Focusing and the Rabbit Tracheal Ciliary Bioassay / Wilson, G. B. / Monsher, M. T. / Arnaud, P. / Fudenberg, H. H. -- Microheterogeneity of Human Kininogen, Alpha- 2-HS and Alpha-1-Acid Glycoproteins by Thin- Layer Isoelectric Focusing / Hamberg, U. / Turpeinen, U. / Knuutinen, U. -- A Genetic Variant of Amylase from Human Parotid Saliva Detected by Isoelectric Focusing / Pronk, J. C. -- 6. Two-Dimensional Techniques -- Biological Applications of Two-Dimensional Gel Electrophoresis / Ivarie, R. D. / Gelfand, D. H. / Jones, P. P. / O'Farrell, P. Z. / Polisky, B. A. / Steinberg, R. A. / O'Farrell, P. H. -- Plant Proteins Evaluated by Two-Dimensional Methods / Stegemann, H. -- Two-Dimensional Separation of Lymphocyte Microsomal Membrane Proteins: Isoelectric Focusing Linked to SDS-Polyacrylamide Gel Electrophoresis / Kniifermann, H. -- Mammalian Mitochondrial Ribosomes: Two- Dimensional Gel Electrophoresis of the 55 S- and the Corresponding Subunit Proteins / Czempiel, W. -- Crossed Immunoelectrofocusing for Standardization and Characterization of Fungal Antigens / Holmberg, K. / Wadström, T. -- 7. Preparative Separations -- Isoelectric Focusing in Free Water / Denckla, W. D. -- Preparative Electrofocusing in Flat-Beds of Granulated Gel - Methodological Aspects / Winter, A. -- Preparative Scale Purification of Bacterial Enzymes and Toxins by Isoelectric Focusing and Isotachophoresis / Wadstrom, T. / Möllby, R. / Olsson, B. / Söderholm, J. / Smyth, C. J. -- Preparative Zone Convection Electrofocusing in Helical Glass Tubes / Quast, R. -- Chromatofocusing: Isoelectric Focusing on Ion Exchangers in the Absence of an Externally Applied Potential / Sluyterman, L. A. A. E. / Wijdenes, J. -- 8. Separation of Cells -- New Instrumentation and Procedures for the Analysis of Mammalian Cells by Electrophoretic Techniques / Catsimpoolas, N. / Griffith, A. L. -- Continuous-Flow Isoelectric Focusing: Further Studies on Human Red Blood Cells and on the Separation of Rat Liver Light Mitochondrial Fraction / Just, W. W. / Werner, G. -- Preparative Electrofocusing of Native and Modified Living Mammalian Cells in a Stationary Ficoll/ Sucrose Gradient / Manske, W. / Bohn, B. / Brossmer, R. -- Part II. Isotachophoresis -- 1. Analytical and Preparative Methodology -- The Principle of Preparative Capillary Isotachophoresis / Arlinger, L. -- Preparative Capillary Isotachophoresis: Separation of µg Amounts of Some Human Serum Proteins / Moberg, U. / Hjalmarsson, S. G. / Arlinger, L. / Lundin, H. -- Quantitative Determination of Picomoles ADP by Means of Steady State Mixed Zones in Isotachophoresis / Wielders, J. P. M. / Everaerts, F. M. -- Thin-Layer Polyacrylamide Gel Isotachophoresis, Crossed Immuno-Isotachophoresis and Crossed Isotachophoresis-Electrofocusing / Brogren, C. H. -- Isotachophoresis of the Cerebrospinal Fluid (CSF) Proteins: Advantage of Prior Dialysis and its Practical Realization / Delmotte, P. -- 2. Applications -- Plasma Protein Fractionation in Sephadex by Isotachophoresis Using Discrete Spacers / Bier, M. / Kopwillem, A. -- Immuno-lsoelectric Focusing Analysis of Antibodies Fractionated by Isotachophoresis / Peltre, G. / Brogren, C. H. -- Analytical and Preparative Isotachophoresis in Column and Flat-Bed Gels for Fractionation of Antibodies / Brogren, C. H. / Peltre, G. -- Index -- Backmatter