Cytogenetic and molecular study among Hodgkin¿s lymphoma patients

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The book sheds light on genetic alterations caused by chromosomal translocation t (14, 18) (q32, q21) that involves the bcl2 and JH genes in some of Hodgkin's lymphoma Iraqi patients.The detection of t (14, 18) translocation involves the hot spots regions of the bcl2 gene which included the major breakpoints (MBR located in the 3' untranslated region of bcl2 exon 3) and the minor cluster region (MCR located in the 3' region of bcl2 exon 3) on chromosome 18 and JH regions on chromosome 14 by using splicing technique, construct fragments of genes and other types of PCR techniques as well as using FISH (fluorescent in situ hybridization) technique for other chromosomal aberrations in the same patients. The book also highlighted the role of BNRF1 gene of EBV in patients by using real time quantification PCR technique (qRT-PCR).