Bovine Trichomoniasis (Classic Reprint)

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Excerpt from Bovine TrichomoniasisThe failure to find the organism at autopsy might be either because these animals had long been infected and the organism had died by autolysis, or because the disappearance of the cervical seal and the relax ation of the cervix provided conditions under which the organism could not survive.Besides these autopsies, the exudate obtained by catheterizing the cervix in several cases of pyometra was examined. The organism was not observed. In all these cases the cervix was Open. The examination of exudates began on March 11, 1937, but all were negative until May 19, 1937. On this date, rectal examination revealed a pyometra in cow 232, bred November 11, 1936 to bull C. When the cervix was Catheterized, the uterine exudate proved to contain large numbers of Trichomonas foetus.Since the organism cannot withstand adverse conditions, a single nega tive finding is inconclusive. The most reliable materials for examination are the exudate accompanying the abortion of a macerated fetus, and the exudate obtained by catheterizing the cervix in a case of pyometra. If, however, the cervix has been Open for some time, the findings may be negative. Vaginal exudate is unreliable and will be negative even in an infected animal unless the organism has come from the uterus within the preceding 24 hours. Samples of vaginal exudate taken twice a week for several weeks from cows bred to a suspected bull will, however, probably reveal the organism if it is present.The examinations should be made as soon after sampling as possible. The organism will remain Viable in a glass container for as long as a week. The length of time depends on the amount of exudate, the number of organisms, the amount of bacterial contamination, and the tempera ture maintained. If the material is allowed to dry, the trichomonads will die and cannot be identified. Consequently, if the sample has to be mailed to a laboratory, a liberal amount of exudate should be collected - if possi ble, about 2 ounces. Should insufficient exudate be available, it should be diluted with sufficient physiological saline (or with tap water) to pre vent drying before reaching the laboratory. As an excessive amount of bacterial growth will kill the trichomonads rapidly, the material should be maintained below the optimum temperature at which the common bacteria grow.About the PublisherForgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.comThis book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully, any imperfections that remain are intentionally left to preserve the state of such historical works.