Bacteriophages
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This book expands on the previous volumes with new chapters exploring emerging themes and methodologies in bacterial virus research. The chapters in this book are divided into 4 parts and cover topics such as: iron chloride flocculation of bacteriophages from seawater, encapsulation of Listeria phage A511 by alginate, examining genome termini of bacteriophage through high-throughput sequencing, genome sequencing of dsDNA-containing bacteriophages directly from a single plaque, characterizing bacteriophages by biology, taxonomy, and genome analysis, phage genome annotation using the RAST pipeline, and the use of RP4::mini-Mu for gene transfer. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Cutting edge and authoritative, Bacteriophages: Methods and Protocols, Volume III is a valuable resource for both established and novice phage scientists.
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