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Annotated Bibliography of Subtilin

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Excerpt from Annotated Bibliography of Subtilin: Assay, Microbiological Production, Purification and Chemistry, Bacteriology, Pharmacology, Therapeutic Activity, Food Preservation, and Related CompoundsJansen, E. F. And Hirschmann, D. J., whbl. Subtilin - An antibacterial product of Bacillus subtilis. Culturing conditions and properties. Arch. Biochem. -309, B. Subtilis (atcc 6633) was grown in surface cultures on media containing sucrose, mineral salts, and several organic nitrogen sources including asparagine, glutamic acid, aspartic acid, casein, and tryptone. The addition of manganese (1 ppm.) proved essential for high antibiotic activity. Wide variations were noted in semi-quantitative serial dilution tests of anti bacterial activity against Stapglococcus aureus Micrococcus conglomeratus (my), and Lactobacillus casei (atcc 70119) of cultures acidified to ph and sterilized for 10 minutes at 10 lbs. Of steam pressure. The material having antibacterial activity was named subtilin, but was believed to consist of more than one substance. A crude concentrate was prepared by centrifuging and vacumn-drying cultures adjusted to ph The active material was reported to be soluble in aqueous ethanol but not in 95 percent ethanol, to be diffusible, to be heat-labile under alkaline conditions but relatively stable at ph and to be inactivated by light and by formaldehyde. (reprints exhausted.)About the PublisherForgotten Books publishes hundreds of thousands of rare and classic books. Find more at www.forgottenbooks.comThis book is a reproduction of an important historical work. Forgotten Books uses state-of-the-art technology to digitally reconstruct the work, preserving the original format whilst repairing imperfections present in the aged copy. In rare cases, an imperfection in the original, such as a blemish or missing page, may be replicated in our edition. We do, however, repair the vast majority of imperfections successfully, any imperfections that remain are intentionally left to preserve the state of such historical works.
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